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It is used to remove excess primers and dNTPs from a PCR mixture with no interference in downstream applications.
Exo sap. The ExoSAP-IT one-step solution provides a simple way to enzymatically purify a PCR reaction. ExoSAP-IT PCR Product Cleanup Reagent High-quality, accurate results, faster turnaround time It is important to achieve a clean template for your Sanger sequencing reaction without losing precious samples. The reaction setup is complete with one pipetting step, which is followed by two incubations.
ExoSAP-IT Express reagent consists of two hydrolytic enzymes, a modified Exonuclease I and Shrimp Alkaline Phosphatase (SAP), in a specially formulated buffer. ExoSAP-IT single-step PCR cleanup utilizes two hydrolytic enzymes, Exonuclease I and Shrimp Alkaline Phosphatase (SAP), together in a specially formulated buffer, to remove unwanted dNTPs and primers from PCR products. Exonuclease I removes residual single-stranded primers and any extraneous single-stranded DNA produced in the PCR.
Our sequencing Exo-SAP-IT PCR product cleanup reagent provides fast, easy, and convenient solutions for your PCR cleanup needs. EXO-Skin Sap Flow Sensor Patent US B1 After more than 25 years of experience and a worldwide experience with sap flow sensor manufacturing and design, the Dynamax team brings you the latest innovations and sap flow sensor construction advancements. The ExoSAP protocol is the simplest way to clean-up PCR products before sequencing.
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